Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, Y.S.; Fang, X.; Wallace, A.L.; Wu, B.; Liu, W.R.
    A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum (2012), J. Am. Chem. Soc., 134, 2950-2953.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.26 expressed in Escherichia coli BL21 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.26 N346A/C348A the mutant displays specific recognition toward non-canonical amino acids and has a broad substrate spectrum with specific recognition of L-phenylalanine Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl Escherichia coli
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.26 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl
-
 Escherichia coli AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.26 PylRS
-
 Escherichia coli
6.1.1.26 pyrrolysyl-tRNA synthetase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.26 ATP
-
 Escherichia coli